In this work, with two concrete cases (WuXiBodies with asymmetric and symmetric designs, respectively), we showed that Capto MMC ImpRes mixed-mode chromatography can effectively remove LC-missing byproducts as well as aggregates, demonstrating that this resin is a powerful tool for WuXiBody purification. CaptoSP ImpRes is a BioProcess chromatography medium developed for large-scale purification of biopharmaceutical Proteins. Diamond MMC Mustang is a relatively new mixed-mode resin, which mediates both cation exchange and hydrophobic interactions. Read more By combining the high-flow characteristics cappto Capto resin with a small. High-resolution intermediate purification and polishing media based on the well-established Capto platform with traditional ligands. The ability to run at higher flow rates and higher bed heights also increases flexibility in process design. Capto SP ImpRes is a strong cation exchange BioProcess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification. Removal: Comparison of CHT Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes. Capto SP ImpRes is a strong cation exchanger for the high-throughput intermediate purification and polishing steps of a wide range of biomolecules. Nevertheless, WuXiBody's unique design greatly facilitates removal of this type of impurity, which can otherwise be difficult to clear. Life Sciences), or Capto adhere ImpRes Media (GE Healthcare. In order to fine-tune the protein/ligand interaction for optimal aggregate removal, the ligand density of Capto MMC ImpRes has been reduced significantly (25 to 39 µmol/mL) compared to Capto MMC (70 to 90 µmol/mL). Capto MMC ImpRes can be used for initial or final polishing steps in mAb purification processes. Capto MMC ImpRes (Fig 1) is a BioProcess chromatography medium (resin) for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules.
LC-missing species, a byproduct frequently associated with bsAb containing sequence engineered Fab arm, is also identified during WuXiBody production. Capto MMC ImpRes comprises small ( 40 µm) beads relative to the related multimodal cation exchange medium, Capto MMC ( 75 µm). BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. Its key feature is the replacement of one parental antibody's CH1/CL constant region with the T cell receptor (TCR) constant domain, a design aimed at promoting cognate heavy chain (HC)-light chain (LC) pairing. Binding of the MAb, purified from CHO cell supernatant by protein A, to Capto adhere ImpRes was therefore performed in 40 mM sodium phosphate, pH 7.8.WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. The results show that the highest binding capacities were obtained at high pH and low salt concentration, while a shorter residence time reduced the capacity (Figure 1). The influence of pH and NaCl concentration on static binding capacity and dynamic binding capacity of Capto adhere ImpRes was determined in PreDictor™ 96-well filter plates and by design of experiment (DoE) in Tricorn™ 5/50 columns, respectively. Here we describe a workflow for method development of a polishing step for a MAb in B/E mode using Capto adhere ImpRes. Here we describe a workflow for method development of a polishing step for a MAb in B/E mode using Capto adhere ImpRes. Cytiva - 17547003 - Capto Q ImpRes, 1 L High-resolution intermediate purification and polishing based on the well-established Capto platform with traditional. Capto Q ImpRes: Strong anion (quaternary amine group) exchange BioProcess medium Capto SP ImpRes: Strong cation (sulfonate group) exchange BioProcess medium. The medium allows operation in either bind-elute (B/E) or flow-through (FT) modes. One of these options is Capto adhere ImpRes, a cost-effective and flexible multimodal anion exchange chromatography medium designed for high-resolution polishing of MAbs.
Binding Capacity/ml Chromatography Medium. GE Healthcare Life Sciences’ MAb toolbox employs protein A chromatography media (resins) as well as a wide range of options for polishing purification steps. The multimodal anion exchanger ligand of Capto adhere ImpRes is immobilized to the base matrix and interacts with the target molecule through multiple types of interactions. A platform approach for MAb purification is desirable as it saves both time and money in process development.
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